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rabbit anti slc12a2  (Proteintech)


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    Structured Review

    Proteintech rabbit anti slc12a2
    Rabbit Anti Slc12a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+slc12a2/NKCC1+Antibody/pmc11564585-182-38-42
    Average 93 stars, based on 34 article reviews
    rabbit anti slc12a2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Control:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Mutagenesis:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Comparison:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Western Blot:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Phospho-proteomics:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Two Tailed Test:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Inhibition:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Injection:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Immunohistochemical staining:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Staining:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Expressing:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Membrane:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..

    Activation Assay:

    Article Title: Early uneven ear input induces long-lasting differences in left–right motor function
    Article Snippet: Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.Sections were blocked in 10% goat serum (Jackson Immunoresearch Lab, West Grove, PA) in 0.3% Triton-X 100 for 1 hour and incubated overnight at 4°C with primary antibody in blocking solution.. Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).. Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or horseradish peroxidase-conjugated (1:250, Jackson Immunoresearch).Tissues were washed 5 times in PBS and incubated for 1 hour at room temperature with the relevant secondary antibodies in blocking solution: Alexa Fluor-488 or Alexa Fluor-568-conjugated (1:350, Life Technologies) or..



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    Image Search Results


    a Schematic illustration of the functions of siR/RSV@TNP in ChP epithelial cells. Illustration created using Microsoft PowerPoint, incorporating elements (siR/RSV@TNP and SPAK siRNA) adapted from in BioRender. Gao, H. (2025) https://BioRender.com/i60a962 . b The mRNA expression of SPAK after different treatments, n = 4 biological replicates for each group. c Western blot result of SPAK. d Immunofluorescence of SPAK. Scale bar, 20 μm. e Western blot result of SPAK. f , g The mRNA expression of NKCC1 and KCNJ13 after different treatments, n = 4 biological replicates for each group respectively. h Western blot result of p-SPAK, n = 3 biological replicates for each group. i Immunofluorescence of pSPAK. Scale bar, 20 μm. All data are presented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

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    doi: 10.1038/s41467-025-61543-1

    Figure Lengend Snippet: a Schematic illustration of the functions of siR/RSV@TNP in ChP epithelial cells. Illustration created using Microsoft PowerPoint, incorporating elements (siR/RSV@TNP and SPAK siRNA) adapted from in BioRender. Gao, H. (2025) https://BioRender.com/i60a962 . b The mRNA expression of SPAK after different treatments, n = 4 biological replicates for each group. c Western blot result of SPAK. d Immunofluorescence of SPAK. Scale bar, 20 μm. e Western blot result of SPAK. f , g The mRNA expression of NKCC1 and KCNJ13 after different treatments, n = 4 biological replicates for each group respectively. h Western blot result of p-SPAK, n = 3 biological replicates for each group. i Immunofluorescence of pSPAK. Scale bar, 20 μm. All data are presented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

    Article Snippet: The expression of SPAK an NKCC1 protein was detected using the Western Blotting analysis, employing specific antibodies for SPAK (ABclonal, A2275, 1:1000) and NKCC1 (Proteintech, 13884-1-AP, 1:1000).

    Techniques: Expressing, Western Blot, Immunofluorescence, Comparison

    Primers used for real-time quantitative PCR.

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    Article Title: High casein concentration induces diarrhea through mTOR signal pathway inhibition in post-weaning piglets

    doi: 10.3389/fmicb.2024.1430511

    Figure Lengend Snippet: Primers used for real-time quantitative PCR.

    Article Snippet: The antibodies used for protein quantification in our study are as follows: beta-actin (60008-1, 1:5,000, Proteintech), RPS60KB1 (A16968, 1:1,000 ABclonal), SLC9A3 (AB2756978, 1:1,000 ABclonal), SLC12A2 (8351S, 1:2,000 CST), SLC26A3 (5642S, 1,1,000 CST), SLC26A6 (A10323, 1:1,000 ABclonal), and SLC5A1 (A13164, 1:1,000 ABclonal), along with the Horseradish Peroxidase (HRP)-conjugated secondary antibodies.

    Techniques:

    Effect of different protein level diets on the relative gene expression of ion transporter carriers of post-weaning piglets. The relative expression of (A,F) SLC5A1, (B,G) SLC9A3, (C,H) SLC12A2, (D,I) of SLC26A3, and (E,J) SLC26A6. Values are expressed as mean ± SEM, n = 7. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, p > 0.05.

    Journal: Frontiers in Microbiology

    Article Title: High casein concentration induces diarrhea through mTOR signal pathway inhibition in post-weaning piglets

    doi: 10.3389/fmicb.2024.1430511

    Figure Lengend Snippet: Effect of different protein level diets on the relative gene expression of ion transporter carriers of post-weaning piglets. The relative expression of (A,F) SLC5A1, (B,G) SLC9A3, (C,H) SLC12A2, (D,I) of SLC26A3, and (E,J) SLC26A6. Values are expressed as mean ± SEM, n = 7. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, p > 0.05.

    Article Snippet: The antibodies used for protein quantification in our study are as follows: beta-actin (60008-1, 1:5,000, Proteintech), RPS60KB1 (A16968, 1:1,000 ABclonal), SLC9A3 (AB2756978, 1:1,000 ABclonal), SLC12A2 (8351S, 1:2,000 CST), SLC26A3 (5642S, 1,1,000 CST), SLC26A6 (A10323, 1:1,000 ABclonal), and SLC5A1 (A13164, 1:1,000 ABclonal), along with the Horseradish Peroxidase (HRP)-conjugated secondary antibodies.

    Techniques: Gene Expression, Expressing